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分别以潮间带沉积物(IWS)和活性污泥(AS)为接种源构建了2个实验室规模的移动床生物膜反应器(命名为RIWS和RAS),对比分析两者的污水处理性能。研究了两者污染物去除效能、微生物群落组成以及氮代谢途径。在稳定运行阶段，RIWS和RAS均取得了优异的有机污染物去除率(>95.0%),RIWS的TN去除率为(83.7±2.3)%优于RAS的(80.1±3.1)%。宏基因组测序结果显示，RIWS系统物种丰富度及多样性高于RAS。此外，为了进一步解释不同的TN去除效能，进行了氮代谢途径以及优势菌属与功能基因之间的相关性分析，发现RIWS和RAS系统中均存在硝化、反硝化、同化硝酸盐还原和异化硝酸盐还原的氮代谢过程，且反硝化作用在脱氮过程中起到主要作用。综上所述，本研究证明了IWS作为一种新型接种源在市政污水处理中具有巨大应用潜力。
Abstract：Two lab-scale moving bed biofilm reactors(RIWS and RAS), seeded with intertidal wetland sediment(IWS) and activated sludge(AS), were constructed to comparatively analyze their performances in wastewater treatment. The pollutant removal efficiency, microbial communities as well as nitrogen metabolic pathway of IWS and AS were investigated. During the steady state superior organic pollutant removal efficiencies(>95.0%) were achieved in both RIWS and RAS. With regards to TN removal rate, RIWS exhibited a higher removal efficiency(83.7±2.3%) than that of RAS(80.1±3.1%). The metagenomic sequencing results revealed that the richness and diversity of RIWS was higher than that of RAS. Moreover, to illustrate different TN removal performances, nitrogen metabolic pathway and correlation analyses between dominant genera and functional genes were conducted. Several pathways(nitrification, denitrification, assimilation nitrate reduction and dissimilatory nitrate reduction) were found in both RIWS and RAS, and denitrification was found to play an important role in nitrogen reduction. In conclusion, this study proved that as a novel inoculant IWS has great application potential for municipal wastewater treatment.
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基本信息:
中图分类号:X703
引用信息:
[1]张国智,张海峰,张翼,等.潮间带沉积物作为处理市政污水的新型接种源研究[J].青岛理工大学学报,2025,46(03):72-78+95.
基金信息:
山东省自然科学基金青年项目(ZR2020QE236)
2024-01-20
2024
2025-04-30
2025
2024-04-29
2
2025-06-30
2025-06-30